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Sarah Smaga

New paper: Interactions between terminal RNA helices affect the stability of the Escherichia coli large ribosomal subunit

One common way to isolate ribosomes produced in vivo is by installing an MS2 affinity tag in the 16S or 23S ribosomal RNA. RNA tags in the 23S rRNA are typically installed into an extended helix 98, as this has no discernible effect on growth… Read More »New paper: Interactions between terminal RNA helices affect the stability of the Escherichia coli large ribosomal subunit

New paper: Atomistic simulations of the E. coli ribosome provide selection criteria for translationally active substrates

There is great current interest in exploiting ribosome chemistry to generate sequence-defined chemical polymers that extend beyond L-⍺-amino acids to new backbone modifications and polymerization chemistries. Although the E. coli ribosome tolerates certain non-L-⍺-amino acids in vitro, few structural insights are available, and the boundary… Read More »New paper: Atomistic simulations of the E. coli ribosome provide selection criteria for translationally active substrates