Publications

2022

Watson, Z.; Knudson, I.; Ward, F. R.; Miller, S. J.; Cate, J. H.; Schepartz, A.; Abramyan, A. M. Atomistic simulations of the E. coli ribosome provide selection criteria for translationally active substrates. bioRxiv 2022, https://doi.org/10.1101/2022.08.13.503842.

Nissley, A. J.; Penev, P. I.; Watson, Z. L.; Banfield, J. F.; Cate, J. H. D. Rare ribosomal RNA sequences from Archaea stabilize the bacterial ribosome. bioRxiv. 2022, https://doi.org/10.1101/2022.07.15.499945.
Data: A loop sequence analysis

Walker, J. A.; Hamlish, N.; Tytla, A.; Brauer, D. D.; Francis, M. B.; Schepartz, A. Redirecting RiPP biosynthetic enzymes to proteins and backbone-modified substrates. ACS Central Science. 2022, https://doi.org/10.1021/acscentsci.1c01577.
Data: SI PDF | Conformers (MAE format)

Fricke, R.*; Swenson, C.*; Roe, L. T.; Hamlish, N.; Ad, O.; Smaga, S.; Gee, C. L.; Schepartz, A. Orthogonal synthetases for polyketide precursors. bioRxiv. 2022, https://doi.org/10.1101/2022.02.28.482149.

Radford, F.; Elliott, S. D.; Schepartz, A.; Isaacs, F. J. Targeted editing and evolution of engineered ribosomes in vivo by filtered editingNature Communications. 202213 (1), 180. https://doi.org/10.1038/s41467-021-27836-x.
Data: Raw NGS data (GSA ID: CRA005526)

2021

Santiago, S.; Ad, O.; Shah, B.; Zhang, Z.; Zhang, X.; Chatterjee, A.; Schepartz, A. Genetic code expansion in the engineered organism Vmax X2: High yield and exceptional fidelity. ACS Central Science. 2021. https://doi.org/10.1021/acscentsci.1c00499.
Data: SI PDF

Gaffney, S. G.; Smaga, S.; Schepartz, A.; Townsend, J. P. Chemsearch: Collaborative compound libraries with structure-aware browsingBioinformatics Advances. 2021, vbab008. https://doi.org/10.1093/bioadv/vbab008.
Data: Chemsearch github repository

Tharp, J. M.*; Walker, J. A.*; Söll, D.; Schepartz, A. Initiating protein synthesis with noncanonical monomers in vitro and in vivo. In Methods in Enzymology; Academic Press, 2021. https://doi.org/10.1016/bs.mie.2021.05.002.

Tharp, J. M.; Vargas-Rodriguez, O.; Schepartz, A.; Söll, D. Genetic encoding of three distinct noncanonical amino acids using reprogrammed initiator and nonsense codonsACS Chemical Biology. 202116 (4), 766–774. https://doi.org/10.1021/acschembio.1c00120.
Data: SI PDF

2020

Krahn, N.; Tharp, J. M.; Crnković, A.; Söll, D. Engineering aminoacyl-tRNA synthetases for use in synthetic biology. In The Enzymes; Academic Press, 2020. doi:10.1016/bs.enz.2020.06.004.

Watson, Z. L.; Ward, F. R.; Méheust, R.; Ad, O.; Schepartz, A.; Banfield, J. F.; Cate, J. H. D., Structure of the bacterial ribosome at 2 Å resolution. eLife. 2020. doi:10.7554/eLife.60482.
Data: PDB (7K00) | EMDR (22586) | EMPIAR-10509

Walker, A. S.; Russ, W. P.; Ranganathan, R.; Schepartz, A. RNA sectors and allosteric function within the ribosomeProceedings of the National Academy of Sciences. 2020. 117 (33). 19879-19887.
Data: Supplementary Materials | SCA6 script | Ribosomal SCA analysis

Tharp, J. M.; Krahn, N.; Varshney, U.; Söll, D., Hijacking translation initiation for synthetic biology, ChemBioChem. 2020, 21 (10). 1387-1396.

Tharp, J. M.; Ad, O.; Amikura, K.; Ward, F. R.; Garcia, E. M.; Cate, J. H. D.; Schepartz, A.; Söll, D., Initiation of protein synthesis with non-canonical amino acids in vivo. Angewandte Chemie. 2020, 59 (8). 3122-3126.
Data: SI PDF

2019

Ward, F. R.*; Watson, Z. L.*; Ad, O.; Schepartz, A.; Cate, J. H. D., Defects in the assembly of ribosomes selected for β-amino acid incorporation, Biochemistry2019, 58, 4494-4504.
Data: WT 50S (EMD 20853) | P7A7 50S (EMD 20854)

Ad, O.; Hoffman, K. S.; Cairns, A. G.; Featherston, A. L.; Miller, S. J.; Söll, D.; Schepartz, A., Translation of diverse aramid- and 1,3-dicarbonyl-peptides by wild type ribosomes in vitro, ACS Central Science. 2019, 5, 1289–1294.
Data: SI PDF

Schepartz, A.; Townsend, J. P., GEM-NET: Lessons in multi-institution teamwork using collaboration software. ACS Central Science. 2019, 5, 1159–1169.
Data: SI PDF | github - strains | portal | podcast-rss

2018

Hoffman, K. S.; Crnković, A.; Söll, D., Versatility of synthetic tRNAs in genetic code expansion, Genes. 2018, 9, epub.

Vargas-Rodriguez, O.; Sevostyanova, A.; Söll, D.; Crnković, A., Upgrading aminoacyl-tRNA synthetases for genetic code expansion, Current Opinion in Chemical Biology2018, 46, 115-122. 

Arranz-Gibert, P.; Vanderschuren, K.; Isaacs, F. J., Next-generation genetic code expansion, Current Opinion in Chemical Biology. 2018, 46, 203-211.

Barber, K. W.; Muir, P.; Szeligowski, R. V.; Rogulina, S.; Gerstein, M.; Sampson, J. R.; Isaacs, F. J.;
Rinehart, J., Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions, Nature Biotechnology. 2018, 36, 638-644.
Data: Supplementary Materials

Schepartz, A., Foldamers wave to the ribosome, Nature Chemistry. 2018, 10, 377.

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